MRI measurements of reporter-mediated increases in transmembrane water exchange enable detection of a gene reporter
نویسندگان
چکیده
منابع مشابه
Magnetization Transfer Detection of GFP: A New MRI Gene Reporter
for GFP vs. BSA comparison. B. Region based MTR calculations for the different frequency offsets for GFP vs. BSA Protein comparison. P-values <0.01 for 0.5, 1, and 5 kHz C. Pseudocolored pixel by pixel MTR calculation for 1 kHz offset for Actin GFP model. D. Region based MTR calculations for the different frequency offsets for Actin GFP model. P-values <0.05 for 10 kHz, <0.01 for 1 and 5 kHz, a...
متن کاملAgrobacterium-Mediated Transformation of Pomegranate (Punica granatum L.) ‘Yousef Khani’ Using the gus Reporter Gene
In this study, an efficient Agrobacterium-mediated transformation method was developed forpomegranate (Punica granatum L.), a difficult-to-transform plant. In vitro shoot segments wereinoculated with Agrobacterium tumefaciens strain LBA4404 harboring the binary vectorpBI121 carrying the neomycin phosphotransferase (nptII) gene as a selectable marker and β-glucuronidase (gus) gene as a reporter....
متن کاملA New Reporter Gene Technology: Opportunities and Perspectives
The paper summarizes the current status of the reporter gene technology and their basics. Reporter gene technology is widely used to monitor cellular events associated with gene expression and signal transduction. Based upon the splicing of transcriptional control elements to a variety of reporter genes, it “reports” the effects of a cascade of signaling events on gene expression inside cells. ...
متن کاملDevelopment of Timd2 as a reporter gene for MRI
PURPOSE To assess the potential of an MRI gene reporter based on the ferritin receptor Timd2 (T-cell immunoglobulin and mucin domain containing protein 2), using T1- and T2-weighted imaging. METHODS Pellets of cells that had been modified to express the Timd2 transgene, and incubated with either iron-loaded or manganese-loaded ferritin, were imaged using T1- and T2-weighted MRI. Mice were als...
متن کاملMRI reporter genes.
Noninvasive molecular imaging of dynamic processes has benefited tremendously from the use of reporter genes. These genes encode for proteins that emit light, bind radiolabeled probes, or, as covered in this review, modulate MRI contrast. Reporter genes play a pivotal role in monitoring cell trafficking, gene replacement therapy, protein-protein interactions, neuronal plasticity, and embryonic ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature Biotechnology
سال: 2016
ISSN: 1087-0156,1546-1696
DOI: 10.1038/nbt.3714